Natural Micron Pharm Tech


Hinokitiol (CAS: 499-44-5)

Hinokitiol (β-thujaplicin) is a natural monoterpenoid found in the wood of trees in the family Cupressaceae. It is a tropolone derivative and one of the thujaplicins. β-thujaplicin has good antibacterial, moisturizing, and pest-repelling effects. It is a high-safety plant component and can be used as an antibacterial and insect repellent. Hinokitiol has a wide range of biological activities, strong bactericidal ability, fragrant smell, and good effect. It can kill bacteria and microbes in the air, prevent pests from invading the human body, and inhibit human pathogenic bacteria. At present, it has been used in the production of shower gel, cosmetics, medicine, and other products. Hinokitiol has a surprising effect on inhibiting Staphylococcus aureus (MRSA) and also has an inhibitory effect on Chlamydia trachomatis.

Cas Number: 499-44-5

EINECS Number: 207-880-7

Molecular Formula: C10H12O2

Structure Formula: 

Appearance:  Almost white to light yellow powder

Other Name: β-thujaplicin; 2-Hydroxy-4-isopropyl-2,4,6-cycloheptatrien-1-one

Send inquiry now to get free samples and best quotation
Send Enquiry Now

Application of hinokitiol

Hinokitiol has been used in the production of shower gel, cosmetics, medicine, agriculture, and other products. In addition, hinokitiol has outstanding effects in the prevention and control of soil-borne diseases.

1. Skin and Oral Care

β-thujaplicin is used in a range of consumer products for skin care, such as soaps, body lotions, eyelid cleansers, shampoos and hair tonics. Oral care such as toothpaste, and breath sprays.

Very good bactericidal and antibacterial effects. For example, Staphylococcus aureus causes skin carbuncle, acne bacillus causes acne, Malassezia that causes folliculitis and dandruff, Candida albicans that causes fungal vaginitis, and other microorganisms have antibacterial effects. And long-term use will not produce drug resistance.

Hinokitiol has an astringent effect, can promote cell metabolism, has an anti-acne bacillus effect, and can be used in anti-acne products. Moreover, juniper alcohol can absorb ultraviolet rays and play a whitening effect.

Anti-inflammatory effect. Because of its phenolic ketone structure can increase the permeability of blood vessels, it can be used for skin inflammation. After clinical tests, hinokitiol can improve the eczema of most patients, and reduce or disappear the itching.

In addition, juniper alcohol also has the functions of oral antibacterial, halitosis removal, lipase activity inhibition, and weight loss.

2. Insect repellent

The inhibitory effect of hinokitiol on termites that destroy crops was found. It has also been shown to be effective against certain mites (Dermatophex farinae, Tyrophagous spp.) and mosquito larvae (Aedes aegypti, Culex papillose).

3. Food preservatives

In experimental studies, hinokitiol has been shown to combat botrytis cinerea, a necrotic fungus that causes botrytis cinerea in many plant species. Hinokitiol can inhibit food browning by inhibiting browning enzymes, especially inhibiting tyrosinase and other polyphenol oxidases by chelating copper ions. Therefore, hinokitiol is used as a shelf-life extender in food packaging.

4. Wood preservatives

Hinokitiol is found in the heartwood of naturally durable trees belonging to the Cupressaceae family. Due to their fungicidal, insecticidal, and insecticidal activities, these compounds make wood naturally resistant to decay and pests. Therefore hinokitiol has been suggested as a wood preservative.

Parameters of β-thujaplicin

Items Specification
Name Hinokitiol
Other Name beta-thujaplicin; 2-Hydroxy-4-isopropyl-2,4,6-cycloheptatrien-1-one
Brand Natural Micron Pharm Tech
Cas Number 499-44-5
EINECS Number 207-880-7
Appearance Almost white to light yellow powder
Molecular Formula C10H12O2
Structure Formula
Molecular Weight 164.2
Melting Point 48-52℃
Solubility Soluble in DMSO (up to 25 mg/ml) or in Ethanol (up to 25 mg/ml).
Purity(GC) ≥ 99.0%
Boiling point 140°C 10mm Hg(lit.)
Place of Origin China
Shelf Life 2 Years
Package Standard packaging includes the following:1kg net weight aluminum foil bag, 25 kg net weight drum and other package sizes are available upon request.
Storage Store in a well-ventilated, cool, dry area, out of direct sunlight. Keep containers tightly closed when not in use.

What is Hinokitiol?

Hinokitiol, also known as β-thujaplicin (cas:499-44-5), is a natural organic compound.

Hinokitiol is a colorless to pale yellow crystalline solid with a distinct pine-like odor. It is sparingly soluble in water but dissolves readily in organic solvents like ethanol and ether.

Hinokitiol is primarily found in the wood and leaves of trees from the Cupressaceae family, such as the Japanese cypress (Chamaecyparis obtusa) and the western red cedar (Thuja plicata).

One of hinokitiol’s most notable features is its strong antimicrobial activity. its ability to inhibit the growth of various bacteria, fungi, and other microorganisms. it also exhibits antioxidant properties, so it can neutralize harmful free radicals in the body.

Hinokitiol Biological/Chemical Reaction

Hinokitiol has various chemical and biological reactions. The following is the specific content of β-thujaplicin

1. Antimicrobial Activity:

Hinokitiol exhibits strong antimicrobial activity against various microorganisms, including bacteria and fungi. It disrupts the cell membranes of these microorganisms, leading to cell death.

2. Antioxidant Activity:

Hinokitiol acts as an antioxidant by scavenging free radicals and reactive oxygen species (ROS).

3. Metal Chelation:

Hinokitiol has the ability to chelate with metal ions, forming stable complexes.

4. Anti-Inflammatory Effects:

Hinokitiol has been shown to possess anti-inflammatory effects by inhibiting the expression of pro-inflammatory cytokines and enzymes.

5. Enzyme Inhibition:

Hinokitiol can inhibit the activity of certain enzymes, including those involved in inflammatory pathways.

6. Chemical Modifications:

Hinokitiol can undergo various chemical modifications, including esterification and oxidation reactions.

7. Complex Formation:

Hinokitiol can form complexes with other molecules, influencing their chemical and biological behaviors.

8. Reaction with Reactive Oxygen Species (ROS):

Hinokitiol can react with ROS and free radicals, converting them into less harmful compounds.

Uses of Hinokitiol

β-thujaplicin has a wide range of applications across different industries due to its various properties. Some of the prominent uses of β-thujaplicin include:

1. Antimicrobial Agent:

Hinokitiol can be used in personal care products, cosmetics, and pharmaceuticals as a natural alternative to synthetic preservatives.

2. Skin Care and Cosmetics:

Hinokitiol is used in skin care products due to the antibacterial and antioxidant properties of Hinokitiol. Included in creams, lotions, serums and other skin care formulations.

3. Wood Preservation:

Hinokitiol is effective in protecting wood from decay and fungal infestation. It is used in wood preservation treatments for furniture, buildings, and outdoor structures.

4. Anti-Inflammatory Agent:

Hinokitiol’s useful in products designed to alleviate skin irritation, redness, and discomfort.

5. Food and Beverage Preservation:

Hinokitiol can be used as a natural preservative in food and beverages to extend shelf life by inhibiting the growth of microorganisms.

6. Hair Care Products:

Hinokitiol an ingredient in shampoos and hair conditioners, promoting scalp health and preventing dandruff.

7. Medical Applications:

Hinokitiol has potential applications in medical fields such as wound healing and as an ingredient in topical formulations for skin infections.

8. Agricultural and Horticultural Uses:

Hinokitiol can be used in agricultural applications to protect crops from fungal diseases and enhance plant growth.

9. Personal Hygiene Products:

Hinokitiol’s can be utilized in products like hand sanitizers, mouthwashes, and other personal hygiene items.

10. Environmental Applications:

Hinokitiol’s metal chelation properties can be used in environmental remediation to remove heavy metals from water and soil.

11. Fragrance and Perfume Industry:

Hinokitiol’s pleasant odor makes it suitable for use in perfumes, fragrances, and scented products.

12. Insect Repellent:

Hinokitiol has been explored as a natural insect repellent.

Hinokitiol Preparation Method

The preparation of β-thujaplicin involves extraction and isolation from these natural sources. Here’s a general overview of the preparation method:

1. Extraction:

The first step involves the extraction of hinokitiol from plant material. This can be achieved using different methods such as steam distillation, solvent extraction, or supercritical fluid extraction.

2. Isolation and Purification:

After extraction, the obtained oil or extract is usually a mixture of various compounds, including hinokitiol. Isolation and purification are necessary to obtain pure hinokitiol.

3. Characterization:

The isolated hinokitiol is then characterized using various analytical techniques, such as spectroscopy (NMR, IR, UV-Vis), chromatography (HPLC, GC), and mass spectrometry, to confirm its identity and purity.

It’s worth noting that hinokitiol can also be synthesized chemically, though natural extraction is a common method due to its presence in plant sources. certainly. If you need a lot of hinokitiol, please contact us to buy. Natural Micron provides you with free test samples and technical support.

Hinokitiol Safety and Risk

β-thujaplicin is considered to be a safe compound when used in appropriate concentrations and under normal conditions. However, you should still pay attention to safety and avoid risks when using


  1. Skin Sensitivity: Hinokitiol is generally well-tolerated by the skin. It is often used in skincare products。
  2. Eye Irritation: Direct contact with the eyes should be avoided. If contact occurs, rinse the eyes thoroughly with water and seek medical attention if irritation persists.
  3. Allergic Reactions: While allergic reactions to hinokitiol are rare, they can still occur in sensitive individuals.
  4. Oral Consumption: Hinokitiol is used in oral care products like toothpaste and mouthwash. However, ingestion of large quantities should be avoided, as it may cause gastrointestinal discomfort.


  1. Excessive Use: Using products containing very high concentrations of hinokitiol may increase the risk of skin irritation or other adverse reactions.
  2. Photosensitivity: Excessive exposure to sunlight while using products containing hinokitiol may cause skin reactions. If using hinokitiol-containing products, it’s a good practice to apply sunscreen during the day.

Hinokitiol Research and Future Development

Many uses of β-thujaplicin are still being developed. In the future, β-thujaplicin may be used in the following aspects.

1. Antimicrobial Properties:

Hinokitiol has strong antibacterial properties, such as bacteria, fungi, etc. In the future, it can be used as a raw material for products such as household disinfectants.

2. Skin Health and Cosmetics:

Hinokitiol’s ability to protect skin cells from oxidative stress and inflammation could contribute to its incorporation in formulations targeting skin aging, blemishes, and other skin conditions.

3. Oral Care:

In the future, hinokitiol concentrations and formulations could be optimized to enhance oral health benefits such as reducing bacterial growth and preventing gum disease.

4. Medical and Therapeutic Applications:

Research is ongoing to explore hinokitiol’s potential therapeutic applications, including its use in wound healing, inflammation reduction, and as an adjuvant in cancer therapies.

5. Nanotechnology and Drug Delivery:

Hinokitiol’s ability to interact with various molecules makes it an interesting component in nanotechnology and drug delivery systems.

Hinokitiol COA.pdf

Hinokitiol TDS.pdf

Did not find the information you need? Click the button below to contact us! (👇)


There are no reviews yet.

Be the first to review “Hinokitiol (CAS: 499-44-5)”

Your email address will not be published. Required fields are marked *

Don’t hesitate to Contact Us!

Reach out to us today and get a complimentary product sample and consultation.